#Syncronis™ C18 Columns - Thermo Fisher Scientific

 

AIC  Offers  Syncronis™ C18 Columns - Thermo Fisher Scientific

 

Highly Reproducible Separations

·         Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.

Excellent Peak Shapes for Enhanced Resolution and Sensitivity

·         Dense surface coverage of silica minimizes secondary interactions and peak tailing

·         Double endcapped for extra surface coverage

Exceptional Column-to-Column Consistency

·         Manufactured, packed, and tested in ISO-9000-accredited facilities

·         Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications

·         Enhanced automated packing process

Available in Three Particle Sizes

·         1.7 µm for rapid ultrahigh-performance LC (UHPLC) separations, and 3 µm and 5 µm for more traditional HPLC analysis

 

Thermo Scientific Innovative# SureStop 9mm Vials.

Thermo Scientific Innovative SureStop 9mm Vials.

Benefits of SureStop Vials – AVCS Caps

How tight should you screw your cap on the vial? How do you avoid misalignments & cap deformities? Remove all doubt by using SureStop Vials with AVCS Caps. This is why:

1. Prevents cap misalignment
   A leveling feature in the SureStop vial design eliminates cap tilt, which can interfere with autosampler functioning, cause septa deformities, and cause sample evaporation.
2. Ensures optimal degree of tightening, every time
   The SureStop vial finish incorporates a definite stop point that gives you positive-feedback on when the cap is optimally tightened. This eliminates overtightened or under tightened caps, which can interfere with autosampler functioning, and cause septa deformities and sample evaporation.

1. SureStop/AVCS vial self-aligning cap
2. Generic vial with cap tilt
3. Generic vial with dislodged septum
4. Generic vial with deformed cap

3. Reduces autosampler interruption, increases autosampler compatibility
   Optimal vial seal compression of SureStop vial-AVCS cap combination
   1. Make vials more compatible with more autosamplers
   2. Have fewer autosampler interruptions due to mishandled vials and vial deformities
4. Provides an alternative to crimp and snap vials, and bonded caps
   Our tested SureStop vials with AVCS caps offer the performance expected from traditional crimp vials without the need for crimping and decrimping tools. Performance tests reported in this  technical note showed that there was less evaporation compared to a crimp vial. Due to the simplicity, reliability and versatility to use the SureStop/AVCS vial-cap system in both GC and LC instruments, you can understand why some customers prefer the easy-to-use SureStop/AVCS vial-cap system to crimped vials.

Principle, Types and Applications of Chromatography

 

Types and Applications of Chromatography  

As a life Science student or researcher, we need to work on different biomolecules. We may need to separate the biomolecules from the mixture or maybe need to identify them. For the identification and separation of biomolecules, analytical techniques have been invented. Chromatography is one of such analytic method. It is defined as an analytical technique used to separate the components from the mixture and analyze the individual component.

The chromatography method was developed in 1903 by Mikhail Tswett. He used this method to separate the mixture of plants’ pigments. He exploited the natural capillary phenomena of paper and cloth for the separation of pigments. He dissolved the plant pigments in water or alcohol. He brought to paper or cloth into the contact of dissolved pigments. Due to the capillary action, the pigments traveled but not in the same speed. lighter the pigment, the faster it was traveling along with the cloth or paper. Due to the difference in the weight of the pigments, the pigments formed different bands of color. The individual band represents the individual pigment. As a result of the formation of colored bands, Mikhail Tswett named this technique as Chromatography. ‘Chroma’ means color and ‘Graphy’ means writing. In the honor of his contribution of Mikhail Tswett is called as Father of Chromatography.

Principle of Chromatography:

The Chromatography technique works on facts that the sample distribute, separate or interact differently to two immiscible phases (A and B). The extent of interaction with two immiscible phases depends on the chemistry of sample. The formula that derives the relation between the sample and two immiscible phases is

Kd is called as partition or distribution coefficient. It is an equilibrium constant that represents the ratio of the concentration of sample that has been distributed in two immiscible solvents. The Kd values indicate the solubility biomolecules in the selected solvents. Kd value is intensely used in Pharmaceutical Industry.

The Two immiscible phases can be any of the physical state – solid, liquid or gas. The one phase is called as Stationary phase and other is Mobile phase. The stationary phase can be solid or liquid supported in solid surface. The mobile phase can be liquid and gas state.

Immiscible phases in chromatography

Types of Chromatography:

As the research has advanced different types of chromatographic techniques have been developed based on bed design, mechanism of separation, physical state of stationary and mobile phase.

Based on design of bed –

The Chromatography types are planar and column. In planar Chromatography, the stationary phase is present as or on a plane. It can be paper or any other stationary phase like silica or alumina gel soaked or impregnated on solid support (mostly glass or plastic). In column chromatography, the solid stationary phase is filled in a tube or a column. If the stationary phase is in liquid state then it is coated on solid surface. If the column is completely filled with stationary phase then packed column. If the stationary phase is coated only the surface of the walls of column leaving hollow space at the center of column then it is called as open tubular column.

Based on physical state of immiscible phases –

The Chromatography are of three types based on the physical states of two immiscible phases namely Liquid Chromatography (Solid-liquid and liquid-liquid) and Gas Chromatography (Gas-liquid and Gas-solid).

In Solid-Liquid Chromatography, where the stationary phase is solid and mobile is in liquid state. In Liquid-Liquid Chromatography, both the immiscible phases are in liquid state. One of the stationary liquid is supported using solid surface.

In Gas-solid Chromatography, the stationary phase is of solid and mobile phase is gas whereas in Gas-liquid Chromatography, the stationary phase is liquid and mobile phase is gas.

Based on the mechanism of separation:

1. Partition Chromatography: It works on the principle that the individual components of mixture have different affinity towards the two immiscible phases. The difference in the affinity is due to the difference in the physical and chemical properties of individual chemical component of the mixture. Based on the affinity, the components of the mixture forms non covalent bond with two immiscible phases gets distributed or separated.
2. Adsorption Chromatography: It works on the principle that components of mixture have different affinity towards an adsorbent. An adsorbent is a material which adsorbs any substance because of non covalent interaction. Out of the two phases, one phase is an adsorbent example silica gel, alumina gel etc.
3. Size exclusion Chromatography: It separates the components of the mixture on the basis of size and shape. It consist of porous gel like material as a stationary phase and liquid as a mobile phase. The pores of porous gel are available in different sizes. The size of the pores are of similar size to biomolecule’s size. when sample is loaded in column filled with porous gel, the smaller components of the mixture penetrates into pores while the larger molecule remain outside. The larger molecule elute or exit first and allow us to separate the mixture of biomolecules of different sizes.
4. 
   Affinity Chromatography: The Affinity Chromatography exploits the specific relationship of enzyme and substrate. Its column consist of support medium on which the substrate are immobilized. When mixture of enzymes or proteins are flooded in the column, the proteins and enzymes that have binding sites for immobilized substrate with bind to it. Whereas the non binding proteins and enzymes will leave the column. Hence, the components of mixture are separated based on the affinity for substrate.
5. Ion Exchange Chromatography: Ion Exchange Chromatography uses positive and negative ion interaction. This Chromatography is specifically used for separating charged molecules. The column consist of either of ions (positive or negative) immobilized on support media. When mixture of ions is poured in the column, the immobilized ion selectively binds the oppositely
   charged ion and separates it from the mixture.

Applications of Chromatography:

1. It is used for separating the individual components from the mixture.
2. It is used for identifying unknown biomolecules.
3. It is used for quantifying the biomolecule.
4. It is used for the characterization of biomolecules.

Thermo Fisher Chromatography consumables

 

AIC Offers  Thermo Fisher Chromatography consumables 

HPLC, GC, Sample preparation,

Autosampler vials and caps, 

Autosampler well plates and accessories.

Featured sample preparation 

Smart Digest™ Kits

Protein-to-data within an hour.

Fast and reproducible protein digestion for improved sequence coverage and data quality over traditional in-solution digestion.

SOLA™ SPE Fritless Cartridges and Plates

Consistency without blocking.

Obtain greater reproducibility with cleaner, more consistent extracts. Revolutionize SPE with fritless technology. Available in a variety of formats to fit your laboratory’s needs.

Thermo Fisher LHC Finnpipette F2 Pipettes

Thermo Fisher LHC  

Manual and Electronic Pipette, PipetteTips, Microplate dispensers, Automated liquid handling systems.

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Thermo Fisher (Gibco, Invitrogen, ABI, Pierce)

 

Thermo Fisher (Gibco, Invitrogen, ABI, Pierce)- Cell Culture and Molbio Range of Consumables.

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Lipofectamine 3000 Transfection Reagent

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Thermo Fisher (Nunc, Nalgene, Biolite)

 

Thermo Fisher (Nunc, Nalgene, Biolite)- Cell Culture Range of Products.